SDS-PAGE procedure: Application for characterization of new entirely uncharged nucleic acids analogs.

Academic Journal

Pavlova AS; Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russian Federation.
Dyudeeva ES; Department of Natural sciences, Novosibirsk State University, Novosibirsk, Russian Federation.
Kupryushkin MS; Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russian Federation.
Amirkhanov NV; Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russian Federation.
Pyshnyi DV; Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russian Federation.; Department of Natural sciences, Novosibirsk State University, Novosibirsk, Russian Federation.
Pyshnaya IA; Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russian Federation.

Electrophoresis [Electrophoresis] 2018 Feb; Vol. 39 (4), pp. 670-674. Date of Electronic Publication: 2017 Nov 29.

English

SDS-PAGE is considered to be a universal method for size-based separation and analysis of proteins. In this study, we applied the principle of SDS-PAGE to the analysis of new entirely uncharged nucleic acid (NA) analogues, - phosphoryl guanidine oligonucleotides (PGOs). The procedure was also shown to be suitable for morpholino oligonucleotides (PMOs) and peptide nucleic acids (PNAs). It was demonstrated that SDS can establish hydrophobic interactions with these types of synthetic NAs, giving them a net negative charge and thus making these molecules mobile in polyacrylamide slab gels under the influence of an electric field.
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