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Chemical Modifications Influence the Number of siRNA Molecules Adsorbed on Gold Nanoparticles and the Efficiency of Downregulation of a Target Protein.
Publication Type: Academic Journal
Source(s): Nanomaterials (Basel, Switzerland) [Nanomaterials (Basel)] 2022 Dec 14; Vol. 12 (24). Date of Electronic Publication: 2022 Dec 14.
Abstract: Small interfering RNAs (siRNAs) are a powerful tool for specific suppression of protein synthesis in the cell, and this determines the attractiveness of siRNAs as a drug. Low resistance of siRNA to nucleases and inability to enter into target cells are...
Phosphoryl guanidine oligonucleotides as primers for RNA-dependent DNA synthesis using murine leukemia virus reverse transcriptase.
Publication Type: Academic Journal
Source(s): Vavilovskii zhurnal genetiki i selektsii [Vavilovskii Zhurnal Genet Selektsii] 2022 Feb; Vol. 26 (1), pp. 5-13.
Abstract: Modern approaches to the detection and analysis of low-copy-number RNAs are often based on the use of RNA-dependent DNA polymerases, for example, in reverse-transcription PCR. The accuracy and eff iciency of cDNA synthesis in the reverse-transcription ...
A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization.
Publication Type: Academic Journal
Source(s): Nanomaterials (Basel, Switzerland) [Nanomaterials (Basel)] 2021 Oct 20; Vol. 11 (11). Date of Electronic Publication: 2021 Oct 20.
Abstract: There is an urgent need to develop systems for nucleic acid delivery, especially for the creation of effective therapeutics against various diseases. We have previously shown the feasibility of efficient delivery of small interfering RNA by means of go...
An Influence of Modification with Phosphoryl Guanidine Combined with a 2'-O-Methyl or 2'-Fluoro Group on the Small-Interfering-RNA Effect.
Publication Type: Academic Journal
Source(s): International journal of molecular sciences [Int J Mol Sci] 2021 Sep 10; Vol. 22 (18). Date of Electronic Publication: 2021 Sep 10.
Abstract: Small interfering RNA (siRNA) is the most important tool for the manipulation of mRNA expression and needs protection from intracellular nucleases when delivered into the cell. In this work, we examined the effects of siRNA modification with the phosph...
Effect of Fluorescent Labels on DNA Affinity for Gold Nanoparticles.
Publication Type: Academic Journal
Source(s): Nanomaterials (Basel, Switzerland) [Nanomaterials (Basel)] 2021 Apr 29; Vol. 11 (5). Date of Electronic Publication: 2021 Apr 29.
Abstract: Fluorophore (FD) labeling is widely used for detection and quantification of various compounds bound to nanocarriers. The systems, composed of gold nanoparticles (GNPs) and oligonucleotides (ONs) labeled with FDs, have wide applications. Our work was a...
Isolation of Extracellular Vesicles from Biological Fluids via the Aggregation-Precipitation Approach for Downstream miRNAs Detection.
Publication Type: Academic Journal
Source(s): Diagnostics (Basel, Switzerland) [Diagnostics (Basel)] 2021 Feb 24; Vol. 11 (3). Date of Electronic Publication: 2021 Feb 24.
Abstract: Extracellular vesicles (EVs) have high potential as sources of biomarkers for non-invasive diagnostics. Thus, a simple and productive method of EV isolation is demanded for certain scientific and medical applications of EVs. Here we aim to develop a si...
Delivery of mRNA Vaccine against SARS-CoV-2 Using a Polyglucin:Spermidine Conjugate.
Publication Type: Academic Journal
Source(s): Vaccines [Vaccines (Basel)] 2021 Jan 21; Vol. 9 (2). Date of Electronic Publication: 2021 Jan 21.
Abstract: One of the key stages in the development of mRNA vaccines is their delivery. Along with liposome, other materials are being developed for mRNA delivery that can ensure both the safety and effectiveness of the vaccine, and also facilitate its storage an...
Rational Design of Albumin Theranostic Conjugates for Gold Nanoparticles Anticancer Drugs: Where the Seed Meets the Soil?
Publication Type: Academic Journal
Source(s): Biomedicines [Biomedicines] 2021 Jan 13; Vol. 9 (1). Date of Electronic Publication: 2021 Jan 13.
Abstract: Multifunctional gold nanoparticles (AuNPs) may serve as a scaffold to integrate diagnostic and therapeutic functions into one theranostic system, thereby simultaneously facilitating diagnosis and therapy and monitoring therapeutic responses. Herein, al...
Amphiphilic "Like-a-Brush" Oligonucleotide Conjugates with Three Dodecyl Chains: Self-Assembly Features of Novel Scaffold Compounds for Nucleic Acids Delivery.
Publication Type: Academic Journal
Source(s): Nanomaterials (Basel, Switzerland) [Nanomaterials (Basel)] 2020 Sep 29; Vol. 10 (10). Date of Electronic Publication: 2020 Sep 29.
Abstract: The conjugation of lipophilic groups to oligonucleotides is a promising approach for improving nucleic acid-based therapeutics' intracellular delivery. Lipid oligonucleotide conjugates can self-aggregate in aqueous solution, which gains much attention ...
Long-term stability and scale-up of noncovalently bound gold nanoparticle-siRNA suspensions.
Publication Type: Academic Journal
Source(s): Beilstein journal of nanotechnology [Beilstein J Nanotechnol] 2019 Dec 23; Vol. 10, pp. 2568-2578. Date of Electronic Publication: 2019 Dec 23 (Print Publication: 2019).
Abstract: Gold nanoparticles (AuNPs) are a platform for the creation of nanoconstructions that can have a variety of functions, including the delivery of therapeutic nucleic acids. We previously designed a AuNP/small interfering RNA (siRNA) nanoconstruction cons...