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The application of capability indices in the validation of ELISA methodology.
Publication Type: Academic Journal
Source(s): Bioanalysis [Bioanalysis] 2023 Nov; Vol. 15 (21), pp. 1277-1286. Date of Electronic Publication: 2023 Sep 22.
Abstract: Aim: To investigate the impact of sample replication number (duplicate vs triplicate) on the validation of ELISA methodology. Materials & methods: The methodology was validated with reference sample and test sample as an 11-point triplicate dilution se...
Validation Procedures for Quantification of Food Allergens by Enzyme-Linked Immunosorbent Assay (ELISA).
Publication Type: Academic Journal
Source(s): Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2717, pp. 285-304.
Abstract: Enzyme-linked immunosorbent assay (ELISA) is a widely used analytical technique for food allergen detection and quantification. Validating ELISA protocols is important for both assay developers and end users as it ensures method reliability. This chapt...
Pd@Pt nanoparticle-linked immunosorbent assay for quantification of Collagen type II.
Publication Type: Academic Journal
Source(s): Analytica chimica acta [Anal Chim Acta] 2023 Jul 25; Vol. 1266, pp. 341265. Date of Electronic Publication: 2023 Apr 24.
Abstract: The evaluation of specific protein content in engineered tissues provides a gateway for developing regenerative medicine treatments. Since collagen type II, the major component of articular cartilage, is critical for the blossoming field of articular c...
Development of a rapid lateral flow assay for detection of anti-coccidioidal antibodies.
Publication Type: Academic Journal
Source(s): Journal of clinical microbiology [J Clin Microbiol] 2023 Sep 21; Vol. 61 (9), pp. e0063123. Date of Electronic Publication: 2023 Sep 01.
Abstract: Coccidioides spp. are dimorphic fungi that are capable of infecting human and non-human mammals and can cause diverse manifestations of coccidioidomycosis or Valley fever (VF). In combination with clinical symptoms and radiographic findings, antibody-b...
Correlations between three ELISA protocols measurements of RTS,S/AS01-induced anti-CSP IgG antibodies.
Publication Type: Academic Journal
Source(s): PloS one [PLoS One] 2023 May 23; Vol. 18 (5), pp. e0286117. Date of Electronic Publication: 2023 May 23 (Print Publication: 2023).
Abstract: Background: RTS,S/AS01 induced anti-circumsporozoite protein (CSP) IgG antibodies are associated with the vaccine efficacy. There is currently no international standardisation of the assays used in the measurement of anti-CSP IgG antibody concentration...
Brain-derived neurotrophic factor measurements in mouse serum and plasma using a sensitive and specific enzyme-linked immunosorbent assay.
Publication Type: Academic Journal
Source(s): Scientific reports [Sci Rep] 2023 May 12; Vol. 13 (1), pp. 7740. Date of Electronic Publication: 2023 May 12.
Abstract: This study is about the quantification and validation of BDNF levels in mouse serum and plasma using a sensitive immunoassay. While BDNF levels are readily detectable in human serum, the functional implications of these measurements are unclear as BDNF...
An ELISA-based assay for determining haemagglutinin potency in egg, cell, or recombinant protein derived influenza vaccines.
Publication Type: Academic Journal
Source(s): Frontiers in immunology [Front Immunol] 2023 Mar 22; Vol. 14, pp. 1147028. Date of Electronic Publication: 2023 Mar 22 (Print Publication: 2023).
Abstract: Background: The current compendial assay for haemagglutinin antigen potency in influenza vaccine is the single radial immunodiffusion (SRID) which is time consuming and can lead to delays in release of vaccine. We previously described an alternate capt...
Evaluation of an Immunoglobulin E Capture Enzyme-Linked Immunosorbent Assay for the Early Diagnosis of Dengue.
Publication Type: Academic Journal
Source(s): Viral immunology [Viral Immunol] 2023 Mar; Vol. 36 (2), pp. 101-109. Date of Electronic Publication: 2023 Mar 02.
Abstract: Dengue virus (DENV) is the etiological agent of dengue, the most important mosquito-transmitted viral disease of humans worldwide. Enzyme-linked immunosorbent assays (ELISAs) designed to detect DENV IgM are commonly used for dengue diagnosis. However, ...
Nanofluidic analytical system integrated with nanochannel open/close valves for enzyme-linked immunosorbent assay.
Publication Type: Academic Journal
Source(s): Lab on a chip [Lab Chip] 2023 Feb 14; Vol. 23 (4), pp. 727-736. Date of Electronic Publication: 2023 Feb 14.
Abstract: There have been significant advances in the field of nanofluidics, and novel technologies such as single-cell analysis have been demonstrated. Despite the evident advantages of nanofluidics, fluid control in nanochannels for complicated analyses is ext...
Interference in ELISA.
Publication Type: Academic Journal
Source(s): Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2612, pp. 91-99.
Abstract: ELISA is a well-established technique used worldwide to quantify analytes present in a diverse milieu of biological samplings. It is especially important to clinicians who rely on the accuracy and precision of the test to administer patient care. Those...