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CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.
Publication Type: Academic Journal
Source(s): The Journal of cell biology [J Cell Biol] 2023 Mar 06; Vol. 222 (3). Date of Electronic Publication: 2022 Dec 23.
Abstract: In recent years, Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) has emerged as a flexible method that enables semi-automated volume ultrastructural imaging. We present a toolset for adherent cells that enables tracking and finding cells, previ...
Investigations on the light and scanning electron microscopic structure of the lingual papillae in the angora goat (Capra hircus): II. Mechanical papillae.
Publication Type: Academic Journal
Source(s): Anatomia, histologia, embryologia [Anat Histol Embryol] 2023 Mar; Vol. 52 (2), pp. 327-335. Date of Electronic Publication: 2022 Nov 13.
Abstract: This study was conducted to examine the light microscopy (LM) and scanning electron microscopic (SEM) structures of mechanical papillae on the tongue in the Angora goat (Capra hircus). As study materials, the tongues of four adult female Angora goats w...
High-Pressure Freezing Followed by Freeze Substitution: An Optimal Electron Microscope Technique to Study Golgi Apparatus Organization and Membrane Trafficking.
Publication Type: Academic Journal
Source(s): Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2557, pp. 211-223.
Abstract: A major goal of structural biologists is to preserve samples as close to their living state as possible. High-pressure freezing (HPF) is a state-of-art technique that freezes the samples at high pressure (~2100 bar) and low temperature (-196 °C) within...
Near-real-time diagnosis of electron optical phase aberrations in scanning transmission electron microscopy using an artificial neural network.
Publication Type: Academic Journal
Source(s): Ultramicroscopy [Ultramicroscopy] 2023 Mar; Vol. 245, pp. 113663. Date of Electronic Publication: 2022 Dec 18.
Abstract: The key to optimizing spatial resolution in a state-of-the-art scanning transmission electron microscope is the ability to measure and correct for electron optical aberrations of the probe-forming lenses precisely. Several diagnostic methods for aberra...
Quantitative analysis of backscattered-electron contrast in scanning electron microscopy.
Publication Type: Academic Journal
Source(s): Journal of microscopy [J Microsc] 2023 Jan; Vol. 289 (1), pp. 32-47. Date of Electronic Publication: 2022 Oct 28.
Abstract: Backscattered-electron scanning electron microscopy (BSE-SEM) imaging is a valuable technique for materials characterisation because it provides information about the homogeneity of the material in the analysed specimen and is therefore an important te...
Studying on effects of boiling on texture, microstructure and physiochemical properties of sea cucumber body wall and its mechanism using second harmonic generation (SHG) microscopy.
Publication Type: Academic Journal
Source(s): Food chemistry [Food Chem] 2023 Jan 30; Vol. 400, pp. 134055. Date of Electronic Publication: 2022 Sep 05.
Abstract: The structural foundation of texture changes in sea cucumber body wall (SCBW) during boiling was investigated using second harmonic generation (SHG) microscopy for the first time. The results from SHG signal imaging, light microscopy, scanning electron...
Morphological features of the Egyptian Ossimi sheep tongue: New scanning electron microscopic insights into its papillary system adaptations to Egyptian ecological conditions.
Publication Type: Academic Journal
Source(s): Anatomia, histologia, embryologia [Anat Histol Embryol] 2023 Mar; Vol. 52 (2), pp. 262-278. Date of Electronic Publication: 2022 Nov 02.
Abstract: The current study aims to illustrate the gross and scanning electron microscopic characterizations and ultrastructural adaptation of the lingual papillary system of Egyptian Ossimi sheep to Egyptian ecological conditions. The tongue had three regions: ...
Field electron emission from tungsten micro-tips coated with various thicknesses of polystyrene nanolayers: Characteristics & analysis.
Publication Type: Academic Journal
Source(s): Ultramicroscopy [Ultramicroscopy] 2023 Feb; Vol. 244, pp. 113643. Date of Electronic Publication: 2022 Nov 21.
Abstract: This paper studies and analyses the characteristics of cold field electron emission from uncoated polycrystalline tungsten tips before and after being coated with an insulating polystyrene layer of different thicknesses. The process of testing the samp...
Cryo-plasma FIB/SEM volume imaging of biological specimens.
Publication Type: Academic Journal
Source(s): ELife [Elife] 2023 Feb 21; Vol. 12. Date of Electronic Publication: 2023 Feb 21.
Abstract: Serial focussed ion beam scanning electron microscopy (FIB/SEM) enables imaging and assessment of subcellular structures on the mesoscale (10 nm to 10 µm). When applied to vitrified samples, serial FIB/SEM is also a means to target specific structures ...
Correlative light and electron microscopic observation of calcium phosphate particles in a mouse kidney formed under a high-phosphate diet.
Publication Type: Academic Journal
Source(s): Scientific reports [Sci Rep] 2023 Jan 16; Vol. 13 (1), pp. 852. Date of Electronic Publication: 2023 Jan 16.
Abstract: Calcium phosphate forms particles under excessive urinary excretion of phosphate in the kidney. While the formation of calcium phosphate particles (CaPs) has been implicated in the damage to renal tubular cells and renal dysfunction, clarifying the ult...