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ALLOJENİK KÖK HÜCRE NAKİLLERİNDE MİKROBİYOTA ETKİSİ.
Publication Type: Academic Journal
Source(s): Istanbul Tip Fakültesi Dergisi. 2022, Vol. 85 Issue 3, p296-304. 9p.
Abstract: Amaç: Çalışmamızda allo-hematopoetik kök hücre nakli (allo- HKHN) uygulanmış hastaların mikrobiyota analizleri yapılmıştır. Nakile ve tedavilere bağlı olarak değişen mikrobiyota florasının engrafman ve Graft-Versus-Host Hastalığı (GVHH) gelişimi ile il...
OPTIMISED GERMINATION PROTOCOL FOR JACKFRUIT SEEDS AND EVALUATION OF METHODS FOR EXTRACTION OF DNA SUITABLE FOR GENETIC ANALYSIS.
Publication Type: Academic Journal
Source(s): African Crop Science Journal. 2022, Vol. 30 Issue 3, p271-281. 11p.
Abstract: Le jacquier (Artocarpus heterophyllus) est une plante sous-utilisée qui est prometteuse pour freiner la sécurité alimentaire et nutritionnelle en Afrique subsaharienne. Cependant, le niveau élevé de métabolites secondaires dans ses tissus entrave consi...
Combination and improvement of conventional DNA extraction methods in Actinobacteria to obtain high-quantity and high-quality DNA.
Publication Type: Academic Journal
Source(s): Iranian Journal of Microbiology. Apr2022, Vol. 14 Issue 2, p186-193. 8p.
Abstract: Background and Objectives: DNA extraction is an important step of any molecular experiment. DNA could not be easily extracted from members of actinomycetes by the usual methods of lysis. Due to the low efficiency of the conventional DNA extraction meth...
RNASE A ENZYME MODIFICATION OF OPTIMIZED SDS PROTOCOL FOR DNA EXTRACTION SUITABLE FOR REAL-TIME PCR SCREENING OF GMOs.
Publication Type: Academic Journal
Source(s): Macedonian Veterinary Review. Mar2022, Vol. 45 Issue 1, p17-25. 9p.
Abstract: As the number of genetically modified crops increases rapidly, their accurate detection is significant for labelling and safety assessment. Currently, real-time PCR is the "golden standard" method for GMO detection. Hence, extraction of high quality DN...
Disproportionate Distribution of HBV Genotypes A and D and the Recombinant Genotype D/E in the High and Low HBV Endemic Regions of Uganda: A Wake-Up Call for Regional Specific HBV Management.
Publication Type: Academic Journal
Source(s): International Journal of Hepatology. 1/11/2022, p1-15. 15p.
Abstract: Background. Hepatitis B virus (HBV) is the leading cause of liver-related diseases. In Uganda, there is a regional disparity in the HBV burden. Our study was aimed at establishing the circulating genotypes in a low and a high endemic region to give pla...
Paper‐Based Bacterial Lysis Enables Sample‐to‐Answer Home‐based DNA Testing.
Publication Type: Academic Journal
Source(s): Advanced Materials Technologies. Feb2023, Vol. 8 Issue 4, p1-8. 8p.
Abstract: Nucleic acid amplification testing (NAAT) is the gold standard for infectious disease diagnostics. Currently NAATs are mainly limited to centralized laboratories, while paper‐based antigen tests are used for rapid home‐based diagnostics. DNA extraction...
An analysis of data curated from 5 years of identifying human remains.
Publication Type: Academic Journal
Source(s): Journal of Forensic Sciences. Mar2023, Vol. 68 Issue 2, p614-620. 7p.
Abstract: An archive of 5 years of cases involving the identification of human remains was curated, collecting information on: The sample type submitted, the number of STR loci yielding interpretable results, the kinship challenge posed, and the outcome for the ...
Thecochaos is not a myth: study of the genus Thecochaos (Amoebozoa, Discosea) – a rediscovered group of lobose amoeba, with short SSU gene.
Publication Type: Academic Journal
Source(s): Organisms Diversity & Evolution. Mar2023, Vol. 23 Issue 1, p7-24. 18p.
Abstract: Two multinucleate amoebae species, nowadays belonging to the genus Thecochaos, were never seen alive since 1913. Stained preparations deposited with the British Museum of Natural History (London) by E. Penard remain the only material proof of the exist...
Quality analysis of genomic DNA and authentication of fisheries products based on distinct methods of DNA extraction.
Publication Type: Academic Journal
Source(s): PLoS ONE. 2/28/2023, Vol. 17 Issue 2, p1-20. 20p.
Abstract: Molecular genetic techniques are an effective monitoring tool, but high-quality DNA samples are usually required. In this study, we compared three different protocols of DNA extraction: NaCl (saline); phenol-chloroform and commercial kit (Promega)—from...
Development of an array of molecular tools for the identification of khapra beetle (Trogoderma granarium), a destructive beetle of stored food products.
Publication Type: Academic Journal
Source(s): Scientific Reports. 2/27/2023, Vol. 13 Issue 1, p1-11. 11p.
Abstract: Trogoderma granarium Everts, the khapra beetle, native to the Indian subcontinent, is one of the world's most destructive pests of stored food products. Early detection of this pest facilitates prompt response towards the invasion and prevents the need...