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Thermodynamic and kinetic analysis on oligomeric protein dissociation using high-pressure native PAGE velocity method.
Publication Type: Academic Journal
Source(s): Analytical biochemistry [Anal Biochem] 2023 Mar 01; Vol. 664, pp. 115035. Date of Electronic Publication: 2023 Jan 05.
Abstract: High pressure is known to dissociate several oligomeric proteins, and regarded as an important tool to shift the oligomerization equilibrium. Native polyacrylamide gel electrophoresis (native PAGE) at high pressure can characterize the dissociates and ...
Real-time monitoring of polyacrylamide gel electrophoresis by the shadowgraph technique.
Publication Type: Academic Journal
Source(s): Journal of biochemistry [J Biochem] 2023 Feb 03; Vol. 173 (2), pp. 65-72.
Abstract: Polyacrylamide gel electrophoresis (PAGE) with sodium dodecyl sulphate (SDS) and Coomassie brilliant blue (CBB) staining is widely used in protein research and requires time for electrophoresis, staining and destaining. Because the protein bands electr...
Separation and Identification of Native Proteoglycans by Composite Agarose-Polyacrylamide Gel Electrophoresis and Immunoblotting.
Publication Type: Academic Journal
Source(s): Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2619, pp. 187-209.
Abstract: Composite agarose-polyacrylamide gel electrophoresis (CAPAGE) in gels of 1.2% w/v polyacrylamide and 0.6% w/v agarose can be used to examine the heterogeneity of full-length native proteoglycan populations and their fragments in crude tissue extracts, ...
Sample preparation for structural mass spectrometry via polyacrylamide gel electrophoresis.
Publication Type: Academic Journal
Source(s): Methods in enzymology [Methods Enzymol] 2023; Vol. 682, pp. 187-210. Date of Electronic Publication: 2022 Dec 30.
Abstract: Mass spectrometry is an analytical technique that can detect protein molecules with high sensitivity. Its use is not limited to the mere identification of protein components in biological samples, but is recently being utilized for large-scale analysis...
Analysis of Plant L-Cysteine Desulfhydrase (LCD) Isozymes by Non-denaturing Polyacrylamide Gel Electrophoresis.
Publication Type: Academic Journal
Source(s): Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2642, pp. 233-240.
Abstract: Hydrogen sulfide (H 2 S) is a signaling molecule that achieves different regulatory functions in animal and plant cells. The cytosolic enzyme L-cysteine desulfhydrase (LCD; EC 4.4.1.28) catalyzes the conversion of cysteine (L-Cys) to pyruvate and ammon...
Polyacrylamide gel electrophoresis: a versatile tool for the separation of nanoclusters.
Publication Type: Academic Journal
Source(s): BioTechniques [Biotechniques] 2023 Jan; Vol. 74 (1), pp. 51-62. Date of Electronic Publication: 2022 Dec 14.
Abstract: Atomically precise nanoclusters comprising 1-100 atoms have emerged as a new class of nanomaterials with intriguing size-dependent physicochemical properties. The significant changes in the properties of nanoclusters were observed in tailoring the numb...
Blue Native PAGE Analysis of Bacterial Secretion Complexes.
Publication Type: Academic Journal
Source(s): Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1615, pp. 321-351.
Abstract: Bacterial protein secretion systems serve to translocate substrate proteins across up to three biological membranes, a task accomplished by hydrophobic, membrane-spanning macromolecular complexes. The overexpression, purification, and biochemical chara...
Letter to the Editor: Weak-Acidic Clear-Native Polyacrylamide Gel Electrophoresis for the Separation of the Intact Forms of Thylakoid Protein Complexes.
Publication Type: Editorial & Opinion
Source(s): Plant & cell physiology [Plant Cell Physiol] 2022 Jul 14; Vol. 63 (7), pp. 883-885.
Differential Proteome Analysis Using 2D-DIGE.
Publication Type: Academic Journal
Source(s): Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2228, pp. 77-84.
Abstract: Classical 2D-PAGE allows comparison and quantitation of proteomes by visualization of protein patterns using gel stains and comparative image analysis. The introduction of fluorescent reagents for protein labeling (difference in-gel electrophoresis or ...
Comparison of extraction methods and nutritional benefits of proteins of milk and dairy products: A review.
Publication Type: Academic Journal
Source(s): Czech Journal of Food Sciences. 2022, Vol. 40 Issue 5, p331-344. 14p.
Abstract: Proteins are the second most essential macromolecules after nucleic acids. This article aimed at the comparative analysis of extraction methods and nutritional benefits of milk and dairy products. Proteins form the body mass and perform several crucial...