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Stepwise Optimization of Real-Time RT-PCR Analysis.
Publication Type: Academic Journal
Source(s): Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2653, pp. 317-332.
Abstract: Quantitative real-time reverse transcription PCR (qRT-PCR) analysis has been used routinely to quantify gene expression levels. Primer design and the optimization of qRT-PCR parameters are critical for the accuracy and reproducibility of qRT-PCR analys...
Adaptive Filtering Framework to Remove Nonspecific and Low-Efficiency Reactions in Multiplex Digital PCR Based on Sigmoidal Trends.
Publication Type: Academic Journal
Source(s): Analytical chemistry [Anal Chem] 2022 Oct 18; Vol. 94 (41), pp. 14159-14168. Date of Electronic Publication: 2022 Oct 03.
Abstract: Real-time digital polymerase chain reaction (qdPCR) coupled with machine learning (ML) methods has shown the potential to unlock scientific breakthroughs, particularly in the field of molecular diagnostics for infectious diseases. One promising applica...
Direct Real-Time PCR for the Detection and Serotyping of Haemophilus influenzae without DNA Extraction.
Publication Type: Academic Journal
Source(s): Journal of clinical microbiology [J Clin Microbiol] 2022 Apr 20; Vol. 60 (4), pp. e0211121. Date of Electronic Publication: 2022 Mar 21.
Abstract: To monitor the burden and changes in Haemophilus influenzae (Hi) disease, direct real-time PCR (drt-PCR) assays have been developed for Hi detection in monoplex form and its six serotypes in triplex form, directly from cerebrospinal fluid (CSF) specime...
Highly purified DNA-containing cell envelopes from fungi for direct use in PCR.
Publication Type: Academic Journal
Source(s): Analytica chimica acta [Anal Chim Acta] 2023 Sep 08; Vol. 1273, pp. 341528. Date of Electronic Publication: 2023 Jun 15.
Abstract: Efficient DNA sample preparation from fungi with the rigid cell walls is still critical for successful polymerase chain reaction (PCR), one of the basic platforms in molecular diagnostics of fungi, especially in medical mycology. Common methods that in...
A qPCR technology for direct quantification of methylation in untreated DNA.
Publication Type: Academic Journal
Source(s): Nature communications [Nat Commun] 2023 Aug 24; Vol. 14 (1), pp. 5153. Date of Electronic Publication: 2023 Aug 24.
Abstract: DNA methylation is important for gene expression and alterations in DNA methylation are involved in the development and progression of cancer and other major diseases. Analysis of DNA methylation patterns has until now been dependent on either a chemic...
Quantitative multiplex polymerase chain reaction in copies ml-1 linearly correlates with standard urine culture in colonies ml-1 for urinary tract infection (UTI) pathogens.
Publication Type: Academic Journal
Source(s): Letters in applied microbiology [Lett Appl Microbiol] 2023 Aug 02; Vol. 76 (8).
Abstract: Standard urine culture (SUC) is the current standard method for confirmation of a urinary tract infection (UTI). SUC identifies microorganisms in urine samples and semi-quantifies these as colony-forming units (CFUs) ml-1. In contrast, quantitative mul...
Quadruplex Real-Time TaqMan ® RT-qPCR Assay for Differentiation of Equine Group A and B Rotaviruses and Identification of Group A G3 and G14 Genotypes.
Publication Type: Academic Journal
Source(s): Viruses [Viruses] 2023 Jul 26; Vol. 15 (8). Date of Electronic Publication: 2023 Jul 26.
Abstract: Equine rotavirus A (ERVA) is the leading cause of diarrhea in foals, with G3P[12] and G14P[12] genotypes being the most prevalent. Recently, equine G3-like RVA was recognized as an emerging infection in children, and a group B equine rotavirus (ERVB) w...
Precise, wide field, and low-cost imaging and analysis of core-shell beads for digital polymerase chain reaction.
Publication Type: Academic Journal
Source(s): Lab on a chip [Lab Chip] 2023 Jul 25; Vol. 23 (15), pp. 3353-3360. Date of Electronic Publication: 2023 Jul 25.
Abstract: Digital droplet reactors have become a valuable tool for the analysis of single cells, organisms, or molecules by discretising reagents into picolitre or nanolitre volumes. However, DNA-based assays typically require processing of samples on the scale ...
Drug Regulatory-Compliant Validation of a qPCR Assay for Bioanalysis Studies of a Cell Therapy Product with a Special Focus on Matrix Interferences in a Wide Range of Organ Tissues.
Publication Type: Academic Journal
Source(s): Cells [Cells] 2023 Jul 05; Vol. 12 (13). Date of Electronic Publication: 2023 Jul 05.
Abstract: Quantitative polymerase chain reaction (qPCR) has emerged as an important bioanalytical method for assessing the pharmacokinetics of human-cell-based medicinal products after xenotransplantation into immunodeficient mice. A particular challenge in bioa...
Designing highly multiplex PCR primer sets with Simulated Annealing Design using Dimer Likelihood Estimation (SADDLE).
Publication Type: Academic Journal
Source(s): Nature communications [Nat Commun] 2022 Apr 11; Vol. 13 (1), pp. 1881. Date of Electronic Publication: 2022 Apr 11.
Abstract: One major challenge in the design of highly multiplexed PCR primer sets is the large number of potential primer dimer species that grows quadratically with the number of primers to be designed. Simultaneously, there are exponentially many choices for m...