scroll to top
0

Mobile Menu

Header Layout

EBSCO Auth Banner

Let's find your institution. Click here.

Page title

Advanced Search Results For "POLYMERASE chain reaction"

1 - 10 of 687,043 results for
 "POLYMERASE chain reaction"
Results per page:

Direct Real-Time PCR for the Detection and Serotyping of Haemophilus influenzae without DNA Extraction.

Publication Type:Academic Journal

Source(s):Journal of clinical microbiology [J Clin Microbiol] 2022 Apr 20; Vol. 60 (4), pp. e0211121. Date of Electronic Publication: 2022 Mar 21.

Abstract:To monitor the burden and changes in Haemophilus influenzae (Hi) disease, direct real-time PCR (drt-PCR) assays have been developed for Hi detection in monoplex form and its six serotypes in triplex form, directly from cerebrospinal fluid (CSF) specime...

View details

Designing highly multiplex PCR primer sets with Simulated Annealing Design using Dimer Likelihood Estimation (SADDLE).

Publication Type:Academic Journal

Source(s):Nature communications [Nat Commun] 2022 Apr 11; Vol. 13 (1), pp. 1881. Date of Electronic Publication: 2022 Apr 11.

Abstract:One major challenge in the design of highly multiplexed PCR primer sets is the large number of potential primer dimer species that grows quadratically with the number of primers to be designed. Simultaneously, there are exponentially many choices for m...

View details

Simple, Precise, and Less Biased GMO Quantification by Multiplexed Genetic Element-Specific Digital PCR.

Publication Type:Academic Journal

Source(s):Journal of AOAC International [J AOAC Int] 2022 Feb 04; Vol. 105 (1), pp. 159-166.

Abstract:Background: To provide the consumer with choices of genetically modified organisms (GMO) or non-GMO, official food labeling systems were established in many countries. Because the threshold GMO content values were set to distinguish between "non-GMO" a...

View details

Comparison of FACS and PCR for Detection of BCMA-CAR-T Cells.

Publication Type:Academic Journal

Source(s):International journal of molecular sciences [Int J Mol Sci] 2022 Jan 14; Vol. 23 (2). Date of Electronic Publication: 2022 Jan 14.

Abstract:Chimeric-antigen-receptor (CAR)-T-cell therapy is already widely used to treat patients who are relapsed or refractory to chemotherapy, antibodies, or stem-cell transplantation. Multiple myeloma still constitutes an incurable disease. CAR-T-cell therap...

View details

Determining Viability of M. ulcerans by 16S rRNA RT Reverse Transcriptase Real-Time PCR.

Publication Type:Academic Journal

Source(s):Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2387, pp. 81-86.

Abstract:To overcome drawbacks of M. ulcerans culture in terms of incubation time and low sensitivity for the detection of viable bacilli from clinical specimens, a highly sensitive and M. ulcerans-specific RNA-based viability assay was developed. The assay com...

View details

Phosphate-Methylated Oligonucleotides as a Novel Primer for PCR and RT-PCR.

Publication Type:Academic Journal

Source(s):Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2392, pp. 261-273.

Abstract:This chapter introduces neutralized DNA (nDNA) as a novel design for the primers of PCR and RT-PCR by methylating phosphate groups of some oligonucleotides in their structures. It starts with an introduction of the nDNA which possesses an electrically ...

View details

Reverse-transcription PCR increases sensitivity of broad-range fungal detection in bronchoalveolar lavage fluid.

Publication Type:Academic Journal

Source(s):Medical mycology [Med Mycol] 2021 Dec 08; Vol. 60 (1).

Abstract:Broad-range PCR targeting 28S D1-D2 ribosomal DNA (rDNA) identifies numerous fungi but has limited sensitivity in clinical specimens. Ribosomal RNA (rRNA) vastly outnumbers rDNA, suggesting reverse transcription (RT)-PCR could improve detection. Among ...

View details

Evaluation of Sensitivity and Cost-Effectiveness of Molecular Methods for the Co-detection of Waterborne Pathogens in India.

Publication Type:Academic Journal

Source(s):Marine biotechnology (New York, N.Y.) [Mar Biotechnol (NY)] 2021 Dec; Vol. 23 (6), pp. 955-963. Date of Electronic Publication: 2021 Oct 29.

Abstract:Waterborne microbial diseases are regarded as a major public health concern, particularly in nations with poor sanitation, a lack of social awareness, and problems linked with low socioeconomic status. Waterborne pathogen identification using tradition...

View details

SARS-CoV-2 detection in wastewater using multiplex quantitative PCR.

Publication Type:Academic Journal

Source(s):The Science of the total environment [Sci Total Environ] 2021 Nov 25; Vol. 797, pp. 148890. Date of Electronic Publication: 2021 Jul 14.

Abstract:A multiplex reverse transcription quantitative PCR (RT-qPCR)-based method was designed for the simultaneous detection of different SARS-CoV-2 genes. In this study, we used three target genes encoding for the nucleocapsid 1 and 3 (N1, N3), and the spike...

View details

Processing Hundreds of SARS-CoV-2 Samples with an In-House PCR-Based Method without Robotics.

Publication Type:Academic Journal

Source(s):Viruses [Viruses] 2021 Aug 28; Vol. 13 (9). Date of Electronic Publication: 2021 Aug 28.

Abstract:The SARS-CoV-2 pandemic has required the development of multiple testing systems to monitor and control the viral infection. Here, we developed a PCR test to screen COVID-19 infections that can process up to ~180 samples per day without the requirement...

View details

banner_970x250 (970x250)

sponsored