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Advanced Search Results For "Protein Array Analysis"

1 - 10 of 36,614 results for
 "Protein Array Analysis"
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Pro-MAP: a robust pipeline for the pre-processing of single channel protein microarray data.

Publication Type: Academic Journal

Source(s): BMC bioinformatics [BMC Bioinformatics] 2022 Dec 09; Vol. 23 (1), pp. 534. Date of Electronic Publication: 2022 Dec 09.

Abstract: Background: The central role of proteins in diseases has made them increasingly attractive as therapeutic targets and indicators of cellular processes. Protein microarrays are emerging as an important means of characterising protein activity. Their acc...

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Protein Microarrays and their Fabrication.

Publication Type: Academic Journal

Source(s): Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2597, pp. 131-142.

Authors:

Abstract: Protein microarrays are an important tool when analyzing multiple analytes simultaneously. As the human genome contains approximately 20,000 genes, examining the interactions of even just one representative protein for each gene requires a high-through...

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Peptides and Anti-peptide Antibodies for Small- and Medium-Scale Peptide and Anti-peptide Affinity Microarrays.

Publication Type: Academic Journal

Source(s): Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2578, pp. 103-120.

Abstract: This chapter describes the principles for selection of antigenic peptides for the development of anti-peptide antibodies suitable for microarray-based multiplex affinity assays and optional mass spectrometry detection. The methods described here are mo...

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Selymatra: A web application for protein-profiling analysis of mass spectra.

Publication Type: Academic Journal

Source(s): Biotechnology and applied biochemistry [Biotechnol Appl Biochem] 2022 Oct; Vol. 69 (5), pp. 1821-1829. Date of Electronic Publication: 2021 Sep 20.

Abstract: Surface enhanced laser desorption/ionization-time of flight (SELDI-TOF) mass spectrometry is a variant of the matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry. It is used in many cases especially for the analysis...

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A pre-processing pipeline to quantify, visualize, and reduce technical variation in protein microarray studies.

Publication Type: Academic Journal

Source(s): Proteomics [Proteomics] 2022 Feb; Vol. 22 (3), pp. e2100033. Date of Electronic Publication: 2021 Oct 27.

Abstract: Technical variation, or variation from non-biological sources, is present in most laboratory assays. Correcting for this variation enables analysts to extract a biological signal that informs questions of interest. However, each assay has different sou...

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RPPA SPACE: an R package for normalization and quantitation of Reverse-Phase Protein Array data.

Publication Type: Academic Journal

Source(s): Bioinformatics (Oxford, England) [Bioinformatics] 2022 Nov 15; Vol. 38 (22), pp. 5131-5133.

Abstract: Summary: Reverse-Phase Protein Array (RPPA) is a robust high-throughput, cost-effective platform for quantitatively measuring proteins in biological specimens. However, converting raw RPPA data into normalized, analysis-ready data remains a challenging...

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Autoantibody signatures discovered by HuProt protein microarray to enhance the diagnosis of lung cancer.

Publication Type: Academic Journal

Source(s): Clinical immunology (Orlando, Fla.) [Clin Immunol] 2023 Jan; Vol. 246, pp. 109206. Date of Electronic Publication: 2022 Dec 15.

Authors:

Abstract: This study aims to discover novel autoantibodies against tumor-associated antigens (TAAs) and establish diagnostic models for assisting in the diagnosis of lung cancer and discrimination of pulmonary nodules (PNs). Ten autoantibodies to TAAbs (TAAbs) w...

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Low-Cost Peptide Microarrays for Mapping Continuous Antibody Epitopes.

Publication Type: Academic Journal

Source(s): Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2578, pp. 63-81.

Abstract: Understanding antibody specificity and defining response profiles to antigens continue to be essential to both vaccine research and therapeutic antibody development. Peptide scanning assays enable mapping of continuous epitopes in order to delineate an...

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A reverse phase protein array based phospho-antibody characterization approach and its applicability for clinical derived tissue specimens.

Publication Type: Academic Journal

Source(s): Scientific reports [Sci Rep] 2022 Dec 26; Vol. 12 (1), pp. 22373. Date of Electronic Publication: 2022 Dec 26.

Authors:

Abstract: Systematic quantification of phosphoprotein within cell signaling networks in solid tissues remains challenging and precise quantification in large scale samples has great potential for biomarker identification and validation. We developed a reverse ph...

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Current advances in antibody-based serum biomarker studies: From protein microarray to phage display.

Publication Type: Academic Journal

Source(s): Proteomics. Clinical applications [Proteomics Clin Appl] 2022 Nov; Vol. 16 (6), pp. e2100098. Date of Electronic Publication: 2022 Sep 27.

Authors:

Abstract: Purpose: This review aims to summarize the technological advances in the field of antibody-based biomarker studies by proteome microarray and phage display. In addition, the possible development directions of this field are also discussed.Experimental ...

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