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Role of three-dimensional cell culture in therapeutics and diagnostics: an updated review.
Publication Type: Academic Journal
Source(s): Drug delivery and translational research [Drug Deliv Transl Res] 2023 Sep; Vol. 13 (9), pp. 2239-2253. Date of Electronic Publication: 2023 Mar 27.
Abstract: Drug development and testing are a tedious and expensive process with a high degree of uncertainty in the clinical success and preclinical validation of manufactured therapeutic agents. Currently, to understand the drug action, disease mechanism, and d...
A rapid MALDI-TOF mass spectrometry-based method for categorization of Pseudomonas aeruginosa from blood cultures as susceptible or resistant to meropenem.
Publication Type: Academic Journal
Source(s): Journal of microbiological methods [J Microbiol Methods] 2023 Sep; Vol. 212, pp. 106803. Date of Electronic Publication: 2023 Aug 06.
Abstract: We developed and evaluated a simple, low-cost matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS)-based method for the detection of meropenem (MER) resistance in Pseudomonas aeruginosa directly from blood. A volume of 50 μL of ...
Cell culture media dependent in vitro dynamics and culture characteristics of adult caprine dermal fibroblast cells.
Publication Type: Academic Journal
Source(s): Scientific reports [Sci Rep] 2023 Aug 22; Vol. 13 (1), pp. 13716. Date of Electronic Publication: 2023 Aug 22.
Abstract: The enhanced availability of functional fibroblasts from precious tissue samples requires an ideal cell-culture system. Therefore, this study was designed to investigate the performance of caprine adult fibroblast cells (cadFibroblast) when cultivated ...
Applications and sensory utilizations of magnetic levitation in 3D cell culture for tissue Engineering.
Publication Type: Academic Journal
Source(s): Molecular biology reports [Mol Biol Rep] 2023 Aug; Vol. 50 (8), pp. 7017-7025. Date of Electronic Publication: 2023 Jun 28.
Abstract: 3D cell culture approaches are cell culture methods that provide good visualization of interactions between cells while preserving the natural growth pattern. In recent years, several studies have managed to implement magnetic levitation technology on ...
Liquid storage of porcine in vitro -produced blastocysts; a practical approach for short storage.
Publication Type: Academic Journal
Source(s): Zygote (Cambridge, England) [Zygote] 2023 Oct; Vol. 31 (5), pp. 441-450. Date of Electronic Publication: 2023 Jun 08.
Abstract: Commercial application of embryo transfer in pig breeding is dependent on the storage of embryos. The aim of this study was to assess the embryo quality of in vitro -produced blastocysts after 3 h liquid storage at 37°C in CO 2 -free medium by evaluati...
"Organized stress" for robust scale-up of intensified production process with fed-batch seed bioreactor.
Publication Type: Academic Journal
Source(s): Biotechnology and bioengineering [Biotechnol Bioeng] 2023 Sep; Vol. 120 (9), pp. 2509-2522. Date of Electronic Publication: 2023 Apr 07.
Abstract: Process intensification has been widely used for many years in the mammalian biomanufacturing industry to increase productivity, agility and flexibility while reducing production costs. The most commonly used intensified processes are operated using a ...
Addressing amino acid-derived inhibitory metabolites and enhancing CHO cell culture performance through DOE-guided media modifications.
Publication Type: Academic Journal
Source(s): Biotechnology and bioengineering [Biotechnol Bioeng] 2023 Sep; Vol. 120 (9), pp. 2542-2558. Date of Electronic Publication: 2023 Apr 25.
Abstract: Previously, we identified six inhibitory metabolites (IMs) accumulating in Chinese hamster ovary (CHO) cultures using AMBIC 1.0 community reference medium that negatively impacted culture performance. The goal of the current study was to modify the med...
COMPARISON OF 2D AND 3D PRIMARY CELL CULTURES OBTAINED FROM EXPLANT OF HIGH-GRADE UROTHELIAL BLADDER CANCER.
Publication Type: Academic Journal
Source(s): Experimental oncology [Exp Oncol] 2023 Jun 26; Vol. 45 (1), pp. 130-136. Date of Electronic Publication: 2023 Jun 26.
Abstract: Studying the biological characteristics of bladder cancer in primary culture can be an effective way for diagnostic and prognostic purposes, as well as choosing a scheme for personalized therapy.Aim: To characterize and compare 2D and 3D primary cell c...
Employing active learning in the optimization of culture medium for mammalian cells.
Publication Type: Academic Journal
Source(s): NPJ systems biology and applications [NPJ Syst Biol Appl] 2023 May 30; Vol. 9 (1), pp. 20. Date of Electronic Publication: 2023 May 30.
Abstract: Medium optimization is a crucial step during cell culture for biopharmaceutics and regenerative medicine; however, this step remains challenging, as both media and cells are highly complex systems. Here, we addressed this issue by employing active lear...
A native multi-dimensional monitoring workflow for at-line characterization of mAb titer, size, charge, and glycoform heterogeneities in cell culture supernatant.
Publication Type: Academic Journal
Source(s): Journal of chromatography. A [J Chromatogr A] 2023 May 10; Vol. 1696, pp. 463983. Date of Electronic Publication: 2023 Apr 07.
Abstract: With growing maturity of the biopharmaceutical industry, new modalities entering the therapeutic design space and increasing complexity of formulations such as combination therapy, the demands and requirements on analytical workflows have also increase...